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human lung epithelial cells  (ATCC)


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    Structured Review

    ATCC human lung epithelial cells
    Human Lung Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 32067 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lung+epithelial+cells/A549/pm42312865-51-14-19
    Average 99 stars, based on 32067 article reviews
    human lung epithelial cells - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: A study on antimicrobial activity of lysine-like peptoids for the development of new antimicrobials.
    Article Snippet: MIC Minimum Inhibitory Concentration HPLC High-Performance Liquid Chromatography MALDI-TOF Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight ATCC American Type Culture Collection LB Luria–Bertani PBS Phosphate-Buffered Saline MHB Mueller-Hinton Broth O.D.. Optical Density IMDM Iscove’s Modified Dulbecco’s HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid DMEM Dulbecco’s Modified Eagle Medium TAMRA 5-carboxytetramethylrhodamine FACS Fluorescence-Activated Cell Sorting DHE Dhydroethidium ROS Reactive Oxygen Species Abbreviations AMR Antimicrobial Resistance MRSA Methicillin-Resistant Staphylococcus Aureus AMP Antimicrobial Peptides CPPs Cell Penetrating Peptides TOP Tosyl-Octamer peptoid MSSA Methicillin-Sensitive Staphylococcus Aureus PAO1 Pseudomonas Aeruginosa SR Selectivity Ratio

    Article Title: Granzyme B cleaves tenascin-C to release its C-terminal domain in rheumatoid arthritis
    Article Snippet: Digestion products were collected and stored at –20°C, and nonspecific interaction sites were saturated using 1% bovine serum albumin (MilliporeSigma, diluted in PBS) for 1 hour at 37°C. .. Wells were then washed with PBS, and 5,000 primary human fibroblasts (extracted and isolated at the laboratory) in complete DMEM, 10,000 keratinocytes (HaCaTs, RRID:CVCL_0038) in complete DMEM, or 10,000 lung epithelial cells (Beas2B, ATCC, CRL-3588) in complete DMEM:F12 (Gibco) were cultured on the remaining substratum at 37°C (5% CO 2 ). .. Phase contrast pictures were taken after 72 hours using a digital inverted microscope (EVOS FL, Life Technologies) at 10× original magnification.

    Isolation:

    Article Title: The cytocompatibility of graphene oxide as a platform to enhance the effectiveness and safety of silver nanoparticles through in vitro studies.
    Article Snippet: .. A549, lung epithelial cells, from the American Type Culture Collection (ATCC), catalogue no CCL-185TM, is a cell line isolated from a 58-year- old carcinoma patient. ..

    Article Title: Granzyme B cleaves tenascin-C to release its C-terminal domain in rheumatoid arthritis
    Article Snippet: Digestion products were collected and stored at –20°C, and nonspecific interaction sites were saturated using 1% bovine serum albumin (MilliporeSigma, diluted in PBS) for 1 hour at 37°C. .. Wells were then washed with PBS, and 5,000 primary human fibroblasts (extracted and isolated at the laboratory) in complete DMEM, 10,000 keratinocytes (HaCaTs, RRID:CVCL_0038) in complete DMEM, or 10,000 lung epithelial cells (Beas2B, ATCC, CRL-3588) in complete DMEM:F12 (Gibco) were cultured on the remaining substratum at 37°C (5% CO 2 ). .. Phase contrast pictures were taken after 72 hours using a digital inverted microscope (EVOS FL, Life Technologies) at 10× original magnification.



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    ATCC murine lung epithelial cells
    LNP-mediated delivery of siRNAs into lung <t>epithelial</t> and fibroblast cells effectively reduces mRNA levels both in vitro and in vivo . (A, B) MLE-12 cells and murine lung fibroblasts were treated with negative control (NC), Alox15, or TGF-β1 siRNA for 24 h, followed by treatment with bleomycin for an additional 24 h, then subjected to real-time PCR assay. Fluorescence images of (C) MLE-12 cells and (D) murine lung fibroblasts treated with 50 nM naked siRNA-Cy5 or siRNA-Cy5 encapsulated with LNPs (siRNA@LNP) for 6 h, followed by staining with Hoechst 33342. Scale bar = 20 μm. (E) Flow cytometry analysis showing the mean fluorescent intensity (MFI) of Cy5 positive cells. (F) Mice were injected with saline, naked siRNA, or siRNA@LNP and sacrificed at 24 h after treatment. Cy5 fluorescence signal in the heart, lung, liver, spleen and kidney was measured by The PhotonIMAGER Optima system immediately. Fluorescence intensity in the lung expressed as total radiant efficiency. n = 3 per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scatter plots representing individual mice.
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    97
    ATCC murine lung epithelial cell line mle
    LNP-mediated delivery of siRNAs into lung <t>epithelial</t> and fibroblast cells effectively reduces mRNA levels both in vitro and in vivo . (A, B) MLE-12 cells and murine lung fibroblasts were treated with negative control (NC), Alox15, or TGF-β1 siRNA for 24 h, followed by treatment with bleomycin for an additional 24 h, then subjected to real-time PCR assay. Fluorescence images of (C) MLE-12 cells and (D) murine lung fibroblasts treated with 50 nM naked siRNA-Cy5 or siRNA-Cy5 encapsulated with LNPs (siRNA@LNP) for 6 h, followed by staining with Hoechst 33342. Scale bar = 20 μm. (E) Flow cytometry analysis showing the mean fluorescent intensity (MFI) of Cy5 positive cells. (F) Mice were injected with saline, naked siRNA, or siRNA@LNP and sacrificed at 24 h after treatment. Cy5 fluorescence signal in the heart, lung, liver, spleen and kidney was measured by The PhotonIMAGER Optima system immediately. Fluorescence intensity in the lung expressed as total radiant efficiency. n = 3 per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scatter plots representing individual mice.
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    Image Search Results


    LNP-mediated delivery of siRNAs into lung epithelial and fibroblast cells effectively reduces mRNA levels both in vitro and in vivo . (A, B) MLE-12 cells and murine lung fibroblasts were treated with negative control (NC), Alox15, or TGF-β1 siRNA for 24 h, followed by treatment with bleomycin for an additional 24 h, then subjected to real-time PCR assay. Fluorescence images of (C) MLE-12 cells and (D) murine lung fibroblasts treated with 50 nM naked siRNA-Cy5 or siRNA-Cy5 encapsulated with LNPs (siRNA@LNP) for 6 h, followed by staining with Hoechst 33342. Scale bar = 20 μm. (E) Flow cytometry analysis showing the mean fluorescent intensity (MFI) of Cy5 positive cells. (F) Mice were injected with saline, naked siRNA, or siRNA@LNP and sacrificed at 24 h after treatment. Cy5 fluorescence signal in the heart, lung, liver, spleen and kidney was measured by The PhotonIMAGER Optima system immediately. Fluorescence intensity in the lung expressed as total radiant efficiency. n = 3 per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scatter plots representing individual mice.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Dual knockdown of Alox15 and TGF-β1 by lipid nanoparticle-delivered siRNA in bleomycin-induced pulmonary fibrosis

    doi: 10.1016/j.bbrep.2026.102534

    Figure Lengend Snippet: LNP-mediated delivery of siRNAs into lung epithelial and fibroblast cells effectively reduces mRNA levels both in vitro and in vivo . (A, B) MLE-12 cells and murine lung fibroblasts were treated with negative control (NC), Alox15, or TGF-β1 siRNA for 24 h, followed by treatment with bleomycin for an additional 24 h, then subjected to real-time PCR assay. Fluorescence images of (C) MLE-12 cells and (D) murine lung fibroblasts treated with 50 nM naked siRNA-Cy5 or siRNA-Cy5 encapsulated with LNPs (siRNA@LNP) for 6 h, followed by staining with Hoechst 33342. Scale bar = 20 μm. (E) Flow cytometry analysis showing the mean fluorescent intensity (MFI) of Cy5 positive cells. (F) Mice were injected with saline, naked siRNA, or siRNA@LNP and sacrificed at 24 h after treatment. Cy5 fluorescence signal in the heart, lung, liver, spleen and kidney was measured by The PhotonIMAGER Optima system immediately. Fluorescence intensity in the lung expressed as total radiant efficiency. n = 3 per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scatter plots representing individual mice.

    Article Snippet: Murine lung epithelial cells (MLE-12 cells) were obtained from ATCC (Manassas, VA, USA) and cultured in DMEM/F12 medium (Corning) containing 4% FBS, 0.005 mg/mL insulin, 0.01 mg/mL transferrin, 30 nM sodium selenite, 10 nM hydrocortisone, 10 nM β-estradiol, 10 mM HEPES, 2 mM l -glutamine, 100 U/mL penicillin G, and 100 μg/mL streptomycin.

    Techniques: In Vitro, In Vivo, Negative Control, Real-time Polymerase Chain Reaction, Fluorescence, Staining, Flow Cytometry, Injection, Saline